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44 - MDC - Medical Sciences and Clinical Research

MDC 142 - Western Blot and ELISA

Code	Start Date	Duration	Venue
MDC 142	16 December 2024	5 Days	Istanbul	Registration Form Link
MDC 142	17 February 2025	5 Days	Istanbul	Registration Form Link
MDC 142	14 April 2025	5 Days	Istanbul	Registration Form Link
MDC 142	16 June 2025	5 Days	Istanbul	Registration Form Link
MDC 142	04 August 2025	5 Days	Istanbul	Registration Form Link
MDC 142	29 September 2025	5 Days	Istanbul	Registration Form Link
MDC 142	24 November 2025	5 Days	Istanbul	Registration Form Link
MDC 142	29 December 2025	5 Days	Istanbul	Registration Form Link

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Course Description

A western blot is a biochemical technique with which a specific protein in a mixture of proteins can be detected by means of antibodies. Enzyme-Linked Immuno Sorbent Assay (ELISA) is a quantitative measurement method based on investigating the antigen-antibody relationship and the activity of an enzyme bound to an antibody. This course aims to give the participants theoretical and practical knowledge about Western Blot and ELISA used for different purposes in the R&D laboratory.

Course Objectives

Introducing the concepts of protein determination
Describing the principles of Western Blot and ELISA
Analyzing of protein content
Troubleshooting the most common problems you may encounter with Western Blot and ELISA
Discussing the advantages and disadvantages of Western Blot and ELISA

Who Should Attend?

Professionalsand research assistants working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
Professionals working in the fields of Biochemistry
Professionals working in the fields of Immunology

Course Details/Schedule

Day 1

Introduction to proteins and methods
Preparation of samples
Isolation of proteins from cells and tissues
Spectrophotometry
Measurement of proteins by Bradford method

Day 2

Introduction to ELISA
Preparation solutions of ELISA
Direct, indirect and sandwich ELISA
Determination of the concentration with ELISA
Data analysis and troubleshooting

Day 3

Introduction to SDS-page gel electrophoresis and equipment
Calculation procedures
Preparation of buffers used in SDS-page gel electrophoresis
Preparation of gels for SDS-page
Determination of proteins by gel staining (Coomassie blue)

Day 4

Introduction to Western Blot and equipment
Preparation of buffers used in Western Blot
Western Blot protein transfer
Transferring proteins to the membrane
Incubation with membrane blocking and primary antibody

Day 5

Incubation with secondary antibody
Introduction to microscopy
Protein detection and visualization image acquisition with a microscope
Western Blot troubleshooting

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SAHARA Group Training and Consultancy INC (in Turkish: SAHARA Group Eğitim ve Danışmanlık AŞ)

Tax Office: Beyoğlu (Istanbul/Turkiye,Tax No: 7390525151 (Vergi Dairesi: Beyoğlu (İstanbul), Vergi No: 7390525151)

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44 - MDC - Medical Sciences and Clinical Research

MDC 142 - Western Blot and ELISA

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