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44 - MDC - Medical Sciences and Clinical Research
MDC 145 - Oxidative Stress and Antioxidants
| Code | Start Date | Duration | Venue | |
|---|---|---|---|---|
| MDC 145 | 28 October 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 145 | 25 November 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 145 | 23 December 2024 | 5 Days | Istanbul | Registration Form Link |
Please contact us for fees
Course Description
Antioxidants have developed in the body to prevent the formation of reactive oxygen species and the damage they cause. All cells fight against oxidative stress with a robust defense system. Excessive increase in oxidant substances or decrease in antioxidants in different forms results in oxidative stress. This course aims to give the participants theoretical and practical knowledge about the oxidative stress techniques used for different purposes in clinical and R&D laboratories.
Course Objectives
- Introducing the oxidative stress
- Describing the principles of oxidative stress and antioxidant parameters
- Analyzing oxidative stress and antioxidant parameters
- Troubleshooting the most common problems
- Discussing the advantages and disadvantages of the methods
Who Should Attend?
- Professionals and research assistants working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
- Professionals working in the fields of Biochemistry
- Professionals working in the areas of hospital departments
Course Details/Schedule
Day 1
- Introduction to clinical biochemistry
- Introduction to blood
- Blood collection tubes
- Introduction to samples
- Collection of samples
Day 2
- Introduction to endogen and exogen antioxidants
- Spectrophotometry and different methods
- Preparation of samples and solvents
- Determination of the total antioxidant status and total oxidant status
- Data analysis and calculation
Day 3
- Introduction to free radicals and oxidative stress
- Oxidative stress and diseases
- Oxidative Stress and Cancer
- Introduction to centrifuge device
- Centrifugation and storage of the samples
Day 4
- Introduction to oxidative stress parameters
- Preparation of samples and solvents
- Determination of catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA)
- Data analysis and calculation
- Troubleshooting
Day 5
- Introduction to HPLC
- Preparation of samples and solvents
- Determination of the antioxidants by HPLC device
- Data analysis and troubleshooting
