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44 - MDC - Medical Sciences and Clinical Research

MDC 145 - Oxidative Stress and Antioxidants

Code Start Date Duration Venue
MDC 145 09 October 2023 5 Days Istanbul Registration Form Link
MDC 145 04 December 2023 5 Days Istanbul Registration Form Link
Please contact us for fees


Course Description

Antioxidants have developed in the body to prevent the formation of reactive oxygen species and the damage they cause. All cells fight against oxidative stress with a robust defense system. Excessive increase in oxidant substances or decrease in antioxidants in different forms results in oxidative stress. This course aims to give the participants theoretical and practical knowledge about the oxidative stress techniques used for different purposes in clinical and R&D laboratories. 

Course Objectives

  • Introducing the oxidative stress
  • Describing the principles of oxidative stress and antioxidant parameters 
  • Analyzing oxidative stress and antioxidant parameters
  • Troubleshooting the most common problems 
  • Discussing the advantages and disadvantages of the methods

Who Should Attend?

  • Professionals and  research assistants working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
  • Professionals working in the fields of Biochemistry
  • Professionals working in the areas of hospital departments

Course Details/Schedule

Day 1

  • Introduction to clinical biochemistry 
  • Introduction to blood
  • Blood collection tubes
  • Introduction to samples
  • Collection of samples

Day 2

  • Introduction to endogen and exogen antioxidants
  • Spectrophotometry and different methods
  • Preparation of samples and solvents
  • Determination of the total antioxidant status and total oxidant status
  • Data analysis and calculation 

Day 3

  • Introduction to free radicals and oxidative stress
  • Oxidative stress and diseases
  • Oxidative Stress and Cancer
  • Introduction to centrifuge device
  • Centrifugation and storage of the samples

Day 4

  • Introduction to oxidative stress parameters 
  • Preparation of samples and solvents
  • Determination of catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA)
  • Data analysis and calculation
  • Troubleshooting

Day 5

  • Introduction to HPLC 
  • Preparation of samples and solvents
  • Determination of the antioxidants by HPLC device
  • Data analysis and troubleshooting