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44 - MDC - Medical Sciences and Clinical Research

MDC 145 - Oxidative Stress and Antioxidants

Code	Start Date	Duration	Venue
MDC 145	23 December 2024	5 Days	Istanbul	Registration Form Link
MDC 145	24 February 2025	5 Days	Istanbul	Registration Form Link
MDC 145	21 April 2025	5 Days	Istanbul	Registration Form Link
MDC 145	16 June 2025	5 Days	Istanbul	Registration Form Link
MDC 145	11 August 2025	5 Days	Istanbul	Registration Form Link
MDC 145	06 October 2025	5 Days	Istanbul	Registration Form Link
MDC 145	01 December 2025	5 Days	Istanbul	Registration Form Link

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Course Description

Antioxidants have developed in the body to prevent the formation of reactive oxygen species and the damage they cause. All cells fight against oxidative stress with a robust defense system. Excessive increase in oxidant substances or decrease in antioxidants in different forms results in oxidative stress. This course aims to give the participants theoretical and practical knowledge about the oxidative stress techniques used for different purposes in clinical and R&D laboratories.

Course Objectives

Introducing the oxidative stress
Describing the principles of oxidative stress and antioxidant parameters
Analyzing oxidative stress and antioxidant parameters
Troubleshooting the most common problems
Discussing the advantages and disadvantages of the methods

Who Should Attend?

Professionals and research assistants working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
Professionals working in the fields of Biochemistry
Professionals working in the areas of hospital departments

Course Details/Schedule

Day 1

Introduction to clinical biochemistry
Introduction to blood
Blood collection tubes
Introduction to samples
Collection of samples

Day 2

Introduction to endogen and exogen antioxidants
Spectrophotometry and different methods
Preparation of samples and solvents
Determination of the total antioxidant status and total oxidant status
Data analysis and calculation

Day 3

Introduction to free radicals and oxidative stress
Oxidative stress and diseases
Oxidative Stress and Cancer
Introduction to centrifuge device
Centrifugation and storage of the samples

Day 4

Introduction to oxidative stress parameters
Preparation of samples and solvents
Determination of catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA)
Data analysis and calculation
Troubleshooting

Day 5

Introduction to HPLC
Preparation of samples and solvents
Determination of the antioxidants by HPLC device
Data analysis and troubleshooting

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SAHARA Group Training and Consultancy INC (in Turkish: SAHARA Group Eğitim ve Danışmanlık AŞ)

Tax Office: Beyoğlu (Istanbul/Turkiye,Tax No: 7390525151 (Vergi Dairesi: Beyoğlu (İstanbul), Vergi No: 7390525151)

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44 - MDC - Medical Sciences and Clinical Research

MDC 145 - Oxidative Stress and Antioxidants

Course Description

Course Objectives

Who Should Attend?

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