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44 - MDC - Medical Sciences and Clinical Research
MDC 150 - Basic Molecular Biochemistry
| Code | Start Date | Duration | Venue | |
|---|---|---|---|---|
| MDC 150 | 22 April 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 20 May 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 10 June 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 15 July 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 12 August 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 09 September 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 07 October 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 04 November 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 150 | 30 December 2024 | 5 Days | Istanbul | Registration Form Link |
Please contact us for fees
Course Description
This course examines the fundamental issues of molecular biochemistry, such as what molecular biochemistry is and how it came to be. Explain which experiments are used in molecular biology research. This course aims to give the participants theoretical and practical knowledge about gene expression used for different purposes in the R&D laboratory.
Course Objectives
- Introducing the concepts of DNA
- Describing the principles of basic molecular biology
- Analyzing of DNA and RNA
- Troubleshooting the most common problems you may encounter with PCR
- Discussing the advantages and disadvantages of the methods
Who Should Attend?
- Professionals and research assistants working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
- Professionals working in the fields of Biochemistry
- Professionals working in the areas of Molecular Biochemistry
Course Details/Schedule
Day 1
- Introduction to DNA and RNA
- Nucleic acid isolation methods
- Isolation of DNA from tissues
- Isolation of RNA from tissues
- RNA and DNA quality and quantity determination
Day 2
- Introduction to primers
- Primer design
- Restriction enzymes
- Cutting DNA with restriction enzymes
- Common problems and solutions in cutting DNA with restriction enzymes
Day 3
- Introduction to recombinant DNA I
- Plasmid Cloning
- Plasmid DNA isolation
- Vector preparation
- Causes and solutions for the failure of plasmid cloning
Day 4
- Introduction to recombinant DNA II
- Insertion of the DNA fragment into the plasmid vector
- Ligation
- Transformation
- Colony screening
Day 5
- Introduction to PCR method
- Preparation of buffers used in gel electrophoresis
- Preparation of gels
- Determination of proteins by gel staining (Coomassie blue)
- DNA detection and visualization image
