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44 - MDC - Medical Sciences and Clinical Research

MDC 150 - Basic Molecular Biochemistry

Code	Start Date	Duration	Venue
MDC 150	07 October 2024	5 Days	Istanbul	Registration Form Link
MDC 150	04 November 2024	5 Days	Istanbul	Registration Form Link
MDC 150	30 December 2024	5 Days	Istanbul	Registration Form Link

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Course Description

This course examines the fundamental issues of molecular biochemistry, such as what molecular biochemistry is and how it came to be. Explain which experiments are used in molecular biology research. This course aims to give the participants theoretical and practical knowledge about gene expression used for different purposes in the R&D laboratory.

Course Objectives

Introducing the concepts of DNA
Describing the principles of basic molecular biology
Analyzing of DNA and RNA
Troubleshooting the most common problems you may encounter with PCR
Discussing the advantages and disadvantages of the methods

Who Should Attend?

Professionals and research assistants working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
Professionals working in the fields of Biochemistry
Professionals working in the areas of Molecular Biochemistry

Course Details/Schedule

Day 1

Introduction to DNA and RNA
Nucleic acid isolation methods
Isolation of DNA from tissues
Isolation of RNA from tissues
RNA and DNA quality and quantity determination

Day 2

Introduction to primers
Primer design
Restriction enzymes
Cutting DNA with restriction enzymes
Common problems and solutions in cutting DNA with restriction enzymes

Day 3

Introduction to recombinant DNA I
Plasmid Cloning
Plasmid DNA isolation
Vector preparation
Causes and solutions for the failure of plasmid cloning

Day 4

Introduction to recombinant DNA II
Insertion of the DNA fragment into the plasmid vector
Ligation
Transformation
Colony screening

Day 5

Introduction to PCR method
Preparation of buffers used in gel electrophoresis
Preparation of gels
Determination of proteins by gel staining (Coomassie blue)
DNA detection and visualization image

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SAHARA Group Training and Consultancy INC (in Turkish: SAHARA Group Eğitim ve Danışmanlık AŞ)

Tax Office: Beyoğlu (Istanbul/Turkiye,Tax No: 7390525151 (Vergi Dairesi: Beyoğlu (İstanbul), Vergi No: 7390525151)

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44 - MDC - Medical Sciences and Clinical Research

MDC 150 - Basic Molecular Biochemistry

Course Description

Course Objectives

Who Should Attend?

Course Details/Schedule

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