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44 - MDC - Medical Sciences and Clinical Research
MDC 152 - Cytotoxicty, Genotoxicity and Apoptosis
| Code | Start Date | Duration | Venue | |
|---|---|---|---|---|
| MDC 152 | 29 April 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 27 May 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 24 June 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 22 July 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 19 August 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 16 September 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 14 October 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 11 November 2024 | 5 Days | Istanbul | Registration Form Link |
| MDC 152 | 02 December 2024 | 5 Days | Istanbul | Registration Form Link |
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Course Description
This course discusses the fundamental aspects of cell culture, emphasizing how cell structure and function are a vital starting points in the in vitro setting. In addition, it examines the basics of in vitro experiments, what kind of research is done in cell culture. Therefore, this course aims to give the participants theoretical and practical knowledge about the cytotoxicity, genotoxicity and apoptosis at cell culture techniques used for different purposes in the R&D cell culture laboratory.
Course Objectives
- Introducing the concepts of cell culture
- Describing the principles of cell culture techniques
- Analyzing cell culture techniques
- Analyzing cytotoxicity methods
- Analyzing genotoxicty methods
- Analyzing apoptosis methods
- Understanding the work of devices in cell culture
- Discussing the details of the practical experience about the taught methods
Who Should Attend?
- Professionals, research assistants and students working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
- Professionals and students working in the fields of Biochemistry
- Professionals and students working in the field of cell culture
Course Details/Schedule
Day 1
- Introduction to cell culture
- Sterilization of workflow
- Advantages and disadvantages of cell culture
- Experiment design in cell culture
- Preparation of media
Day 2
- Introduction to the biology of cells in culture
- Analysis of the morphology of cells
- Freezing and thawing of cells
- Trypsinization procedure
- Counting of cells by hemocytometer
Day 3
- Introduction to cell quantification, cytotoxicity and viability, intracellular ROS (CYTOTOXICTY)
- Seeding of cells in 96-well and 6-well plates
- Cell viability methods
- Determination of cell viability
- Data analysis
Day 4
- Introduction to apoptosis and autophagy of cells (APOPTOSIS)
- Apoptosis by Annexin V
- Apoptosis by acridine orange/ethidium bromide stain
- Image acquisition with a fluorescent microscope
- Data analysis and troubleshooting
Day 5
- Introduction to Genotoxicity (DNA DAMAGE)
- DNA damage by Comet Assay method
- Image acquisition with a fluorescent microscope
- Data analysis and calculation of DNA tail
- Troubleshooting
