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44 - MDC - Medical Sciences and Clinical Research

MDC 152 - Cytotoxicty, Genotoxicity and Apoptosis

Code	Start Date	Duration	Venue
MDC 152	14 October 2024	5 Days	Istanbul	Registration Form Link
MDC 152	11 November 2024	5 Days	Istanbul	Registration Form Link
MDC 152	02 December 2024	5 Days	Istanbul	Registration Form Link

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Course Description

This course discusses the fundamental aspects of cell culture, emphasizing how cell structure and function are a vital starting points in the in vitro setting. In addition, it examines the basics of in vitro experiments, what kind of research is done in cell culture. Therefore, this course aims to give the participants theoretical and practical knowledge about the cytotoxicity, genotoxicity and apoptosis at cell culture techniques used for different purposes in the R&D cell culture laboratory.

Course Objectives

Introducing the concepts of cell culture
Describing the principles of cell culture techniques
Analyzing cell culture techniques
Analyzing cytotoxicity methods
Analyzing genotoxicty methods
Analyzing apoptosis methods
Understanding the work of devices in cell culture
Discussing the details of the practical experience about the taught methods

Who Should Attend?

Professionals, research assistants and students working in the medical fields (Medicine, Pharmacy, Dentistry, Veterinary Medicine and Basic Sciences)
Professionals and students working in the fields of Biochemistry
Professionals and students working in the field of cell culture

Course Details/Schedule

Day 1

Introduction to cell culture
Sterilization of workflow
Advantages and disadvantages of cell culture
Experiment design in cell culture
Preparation of media

Day 2

Introduction to the biology of cells in culture
Analysis of the morphology of cells
Freezing and thawing of cells
Trypsinization procedure
Counting of cells by hemocytometer

Day 3

Introduction to cell quantification, cytotoxicity and viability, intracellular ROS (CYTOTOXICTY)
Seeding of cells in 96-well and 6-well plates
Cell viability methods
Determination of cell viability
Data analysis

Day 4

Introduction to apoptosis and autophagy of cells (APOPTOSIS)
Apoptosis by Annexin V
Apoptosis by acridine orange/ethidium bromide stain
Image acquisition with a fluorescent microscope
Data analysis and troubleshooting

Day 5

Introduction to Genotoxicity (DNA DAMAGE)
DNA damage by Comet Assay method
Image acquisition with a fluorescent microscope
Data analysis and calculation of DNA tail
Troubleshooting

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SAHARA Group Training and Consultancy INC (in Turkish: SAHARA Group Eğitim ve Danışmanlık AŞ)

Tax Office: Beyoğlu (Istanbul/Turkiye,Tax No: 7390525151 (Vergi Dairesi: Beyoğlu (İstanbul), Vergi No: 7390525151)

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44 - MDC - Medical Sciences and Clinical Research

MDC 152 - Cytotoxicty, Genotoxicity and Apoptosis

Course Description

Course Objectives

Who Should Attend?

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