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45 - PHR - Pharmaceutical Sciences

PHR 110 - Introduction to Cell Culture

Code	Start Date	Duration	Venue
PHR 110	28 October 2024	5 Days	Istanbul	Registration Form Link
PHR 110	02 December 2024	5 Days	Istanbul	Registration Form Link

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Course Description

Cell culture has become one of the major tools used in science today. This introductory course aims to discuss the fundamentals of a cell culture laboratory. Participants are expected to gain a comprehensive knowledge on cell biology and laboratory procedure including the needed conditions and techniques to maintain the cell lines. Attendees will learn about interpreting the microscopic images and making conscious decisions on maintaining cell lines. This course offers an overview of cell-based assays today, advantages and outcomes. Moreover, novel cell experiment strategies will be discussed.

Course Objectives

Understanding cell biology and their growth in laboratory condition
Requirements of a cell culture laboratory and equipment use
Maintaining cell lines
Overviewing cell-based assays (viability/ cytotoxicity tests, transport studies etc.)
Learning novel cell culture strategies

Who Should Attend?

Research and development person
Professionals working in the fields of Molecular Biology and Bioengineering
Professionals working in the Medical fields (Medicine, Pharmacy, Dentistry and Veterinary Medicine)
Researchers
Lab technicians

Course Details/Schedule

Day 1

History and introduction of cell culture
Equipment of a cell culture laboratory
Aseptic techniques
Cell culture types (primary and secondary cell cultures)
Development of growth curves

Day 2

Observing media (Composition, preparation and changing of media)
Plating cells from frozen stocks and freezing cells
Adherent and suspension cell growth
Passage/ subcultivation
Inverted microscope (cell image interpretation)

Day 3

Cell counting via Hemocytometer
Viability/ cytotoxicity tests: Trypan Blue, LDH Assay, MTT Assay, Alamar blue
Fluorescence Activated Cell Sorting (FACS)

Day 4

Cell banks and ordering procedure
Types of biological contamination and prevention
Transport studies (Cell signaling and Teer measurement)

Day 5

Uptake and release studies
Basics for protein isolation
Co-culture systems
3D cell cultures
Organ-on-a-chip (OC)

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SAHARA Group Training and Consultancy INC (in Turkish: SAHARA Group Eğitim ve Danışmanlık AŞ)

Tax Office: Beyoğlu (Istanbul/Turkiye,Tax No: 7390525151 (Vergi Dairesi: Beyoğlu (İstanbul), Vergi No: 7390525151)

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45 - PHR - Pharmaceutical Sciences

PHR 110 - Introduction to Cell Culture

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