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44 - PHR - Pharmaceutical Sciences

PHR 110 - Introduction to Cell Culture

Code Start Date Duration Venue
PHR 110 24 April 2023 5 Days Istanbul Registration Form Link
PHR 110 19 June 2023 5 Days Istanbul Registration Form Link
PHR 110 14 August 2023 5 Days Istanbul Registration Form Link
PHR 110 09 October 2023 5 Days Istanbul Registration Form Link
PHR 110 04 December 2023 5 Days Istanbul Registration Form Link
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Course Description

Cell culture has become one of the major tools used in science today. This introductory course aims to discuss the fundamentals of a cell culture laboratory. Participants are expected to gain a comprehensive knowledge on cell biology and laboratory procedure including the needed conditions and techniques to maintain the cell lines. Attendees will learn about interpreting the microscopic images and making conscious decisions on maintaining cell lines. This course offers an overview of cell-based assays today, advantages and outcomes. Moreover, novel cell experiment strategies will be discussed. 

Course Objectives

  • Understanding cell biology and their growth in laboratory condition
  • Requirements of a cell culture laboratory and equipment use
  • Maintaining cell lines
  • Overviewing cell-based assays (viability/ cytotoxicity tests, transport studies etc.)
  • Learning novel cell culture strategies

Who Should Attend?

  • Research and development person
  • Professionals working in the fields of Molecular Biology and Bioengineering
  • Professionals working in the Medical fields (Medicine, Pharmacy, Dentistry and Veterinary Medicine)
  • Researchers
  • Lab technicians

Course Details/Schedule

Day 1

  • History and introduction of cell culture
  • Equipment of a cell culture laboratory
  • Aseptic techniques 
  • Cell culture types (primary and secondary cell cultures)
  • Development of growth curves

Day 2

  • Observing media (Composition, preparation and changing of media)
  • Plating cells from frozen stocks and freezing cells 
  • Adherent and suspension cell growth
  • Passage/ subcultivation
  • Inverted microscope (cell image interpretation)

Day 3

  • Cell counting via Hemocytometer
  • Viability/ cytotoxicity tests: Trypan Blue, LDH Assay, MTT Assay, Alamar blue
  • Fluorescence Activated Cell Sorting (FACS)

Day 4

  • Cell banks and ordering procedure 
  • Types of biological contamination and prevention 
  • Transport studies (Cell signaling and Teer measurement) 

Day 5

  • Uptake and release studies
  • Basics for protein isolation 
  • Co-culture systems
  • 3D cell cultures
  • Organ-on-a-chip (OC)